Photosynthtic rate at light satulation point (1500 PPFD) was measured by using LI-6400. Red-blue light of LI-6400 was used as the light source. CO2 concentration of the reference gas was kept as 370 ppm.
LI-6400, Li-cor, USA
Measurement for light-photosynthetic curve
The light intensity was decreased and photosynthetic rate at each light intensity was measured. At dark condition, the dark respiration was measured.
Amax, Vcmax, initial slope of the light curve, and dark respiration rate were calculated.